Journal: Cell Stem Cell

Article Title: Selective advantage of mutant stem cells in human clonal hematopoiesis is associated with attenuated response to inflammation and aging

doi: 10.1016/j.stem.2024.05.010

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Article Snippet: APC anti-human CD271 (clone ME20.4-1.H4) , Miltenyi , Cat# 130-113-418; RRID: AB_2733363.

Techniques: Recombinant, Reverse Transcription, DNA Library Preparation, DNA Sequencing, RNA Sequencing, Purification, Sequencing, Software, Sterility

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Figure Lengend Snippet: Genetic organization of the 6,388-bp region of φP27 DNA analyzed in this study (A) and comparison with a corresponding region of 9,760 bp of phage 933W (B). The arrows indicate the length and direction of transcription of ORFs. Black arrows indicate homologues DNA sequences of φP27 and 933W. Gray-shaded arrows represent ORFs with homologies to EMBL-GenBank sequences. ORFs labeled with white arrows had no significant homologies to sequences present in database libraries. The ORF labels of the 933W sequence were the same as those used by Plunkett et al. (22). tRNAs are depicted as black rectangles. The DNA regions marked with a lightly shaded rectangle represent 93% sequence identity. The scale is in kilobases.

Article Snippet: Nucleotide sequencing was performed on both strands with an automated DNA sequencer (model 377; Perkin-Elmer/Applied Biosystems, Weiterstadt, Germany) using universal and reverse primers for pUC/M13 vectors and customized primers.

Techniques: Labeling, Sequencing